hot firepol probe qpcr mix plus (rox)  (Solis BioDyne)

Journal: STAR Protocols

Article Title: Protocol for RNA interference in gene expression in adult insects of Triatoma infestans (Hemiptera: Reduviidae)

doi: 10.1016/j.xpro.2025.103865

Figure Lengend Snippet: Relative expression levels of per mRNA in the heads of adult Triatoma infestans after injection with per-specific double-stranded RNA (dsRNA) Levels of per transcript quantification was performed using quantitative PCR (qPCR), normalizing against β - actin mRNA. Results are displayed as boxplots, with the boxes representing the interquartile range and the whiskers indicating data variability outside the upper and lower quartiles. Asterisks indicate statistically significant differences between the dsRNA per group and controls (∗∗∗p <0.001, ∗∗∗∗p < 0.0001). Control group: group non injected; Control β-Lac dsRNA group: group injected with β-Lac dsRNA; dsRNA per group: group injected with per dsRNA.

Article Snippet: HOT FIREPol Probe qPCR Mix Plus (ROX), 5X , SOLIS BIODYNE , Cat#08-14-00001.

Techniques: Expressing, Injection, Real-time Polymerase Chain Reaction, Control

Journal: Journal of fish diseases

Article Title: Effects of Different Preservatives During Ecological Monitoring of Myxozoan Parasite Tetracapsuloides bryosalmonae Causing Proliferative Kidney Disease (PKD) in Salmonids.

doi: 10.1111/jfd.14095

Figure Lengend Snippet: FIGURE 2 | (a) 2% agarose gel electrophoresis of end-point multiplex PCR product. Samples that were preserved in isopropanol are shown with- in rectangles. The ladder ranges from 100 to 1000 bp with the 166 bp PKD-realF/PKD-realR, 298 bp PKX3F/PKX4R and 756 bp PKD-realF/PKX4R T. bryosalmonae amplicons in addition to the 500 bp salmonid target. POS—T. bryosalmonae positive control, TROUT—salmonid positive control. Sample #23 provides an example of where the salmonid amplicon was not present due to DNA degradation yet the shorter T. bryosalmonae amplicons are still present. (b) Effect of preservative on parasite detection using qPCR, mean Ct value of three technical replicates displayed on the y-axis. Less cycles indicate a higher starting quantity of parasite DNA in the fish kidney tissue. Line, box, whiskers and dots represent the median, first to third quartile, variability as 1.5 times interquartile range and outliers respectively.

Article Snippet: The assay consisted of a 10 μL reaction comprised of 4.94 μL dH2O, 2 μL 5× HOT FIREPol Probe qPCR Mix Plus (Solis BioDyne; Tartu, Estonia) and 0.02 μL of forward and reverse primers (PKX18s1266f- 1426r, 91 bp, Hutchins et al. 2018), probe (Taqman double quenched PKX18s_1399probe, Hutchins et al. 2018) (100 pmol μL−1 concentration) and 3 μL of DNA template (20 ng μL−1 concentration). qPCR was conducted on a CFX384 Touch Real- Time PCR Detection System (Bio- Rad Laboratories Inc.; Hercules, CA, USA) with analyses performed in CFX Maestro 2.3 software (Bio- Rad Laboratories Inc.).

Techniques: Agarose Gel Electrophoresis, Multiplex Assay, Positive Control, Amplification